Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human PKCβ around the phosphorylation site of threonine 641 (E-L-TP-P-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityPKCβ (phospho-Thr641) antibody detects endogenous levels of PKCβ only when phosphorylated at threonine 641.
ReferencesZhang Y, et al. (2006) Mol Cell Biol ; 26: 6748-6761
Castoria G, et al. (2004) Mol Cell Biol ; 24: 7643-7653
Marcil J, et al. (1999) Biochem J ; 337:185-192
Bornancin F, et al. (1996) Curr Biol ; 6:1114-1123.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells