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Primary Antibodies

HSP27 (Phospho-Ser15) Antibody

E011164-1Cite ID: EN0000317DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200
P04792
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine15 (G-P-SP-W-D).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityHSP27 (phospho-Ser15) antibody detects endogenous levels of HSP27only when phosphorylated at serine 15.
ReferencesTremolada L, et al. (2005) Proteomics. 5(3): 788-795. McGregor E, et al. (2004) Mol Cell Proteomics. 3(2): 115-124. Benn SC, et al. (2002) Neuron. 36(1): 45-56. MacDonald JA, et al. (2002) Mol Cell Proteomics. 1(4): 314-322.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HSP27 (phospho-Ser15) antibody (E011164).
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HSP27 (phospho-Ser15) antibody (E011164).
Western blot analysis of extract from HeLa cells using HSP27 (Ab-15) antibody (E021144, Lane1 and 2) and HSP27 (phospho-Ser15) antibody (E011164, Lane 3 and 4).
Western blot analysis of extract from HeLa cells using HSP27 (Ab-15) antibody (E021144, Lane1 and 2) and HSP27 (phospho-Ser15) antibody (E011164, Lane 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011164-1-Conjugate. Price: $485 / Size: 100μl.

E011164-1-FITC$485 / 100μlE011164-1-Biotin$485 / 100μlE011164-1-HRP$485 / 100μlE011164-1-AP$485 / 100μlE011164-1-AF350$485 / 100μlE011164-1-AF405M$485 / 100μlE011164-1-AF488$485 / 100μlE011164-1-AF514$485 / 100μlE011164-1-AF532$485 / 100μlE011164-1-AF555$485 / 100μlE011164-1-AF568$485 / 100μlE011164-1-AF405S$485 / 100μlE011164-1-AF405L$485 / 100μlE011164-1-AF546$485 / 100μlE011164-1-AF594$485 / 100μlE011164-1-AF610$485 / 100μlE011164-1-AF635$485 / 100μlE011164-1-AF647$485 / 100μlE011164-1-AF680$485 / 100μlE011164-1-AF700$485 / 100μlE011164-1-AF750$485 / 100μlE011164-1-AF790$485 / 100μlE011164-1-APC$485 / 100μlE011164-1-PE$485 / 100μlE011164-1-Cy3$485 / 100μlE011164-1-Cy5$485 / 100μlE011164-1-Cy5.5$485 / 100μlE011164-1-Cy7$485 / 100μlE011164-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.