All Names
AlternameI-kappa-B-alpha, IKBA, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappaB inhibitor alpha, NFKBI, NFKBIA, RL/IF-1
All SitesHuman: Tyr42; Mouse: Tyr42; Rat: Tyr42
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human IκB-α around the phosphorylation site of tyrosine 42 (E-E-YP-E-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityIκB-α (phospho-Tyr42) antibody detects endogenous levels of IκB-α only when phosphorylated at tyrosine 42.
ReferencesBéraud C, et al. (1999) Proc Natl Acad Sci U S A 96(2): 429-434.
Sundström S, et al. (2005) J Virol 79(4): 2230-2239.
Liu L, et al. (1998) Mol Cell Biol 18(7): 4221-4234.
Shrivastava A, et al. (1998) J Virol 72(12): 9722-9728.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells