Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 493 (S-Y-YP-T-A).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityZap-70 (phospho-Tyr493) antibody detects endogenous levels of Zap-70 only when phosphorylated at tyrosine 493
ReferencesChan AC, et al. (1995) EMBO J; 14(11): 2499-2508
Kong G, et al. (1996) Mol Cell Biol; 16(9): 5026-5035
Salomon AR, et al. (2003)Proc Natl Acad Sci U S A; 100(2): 443-448
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells