Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human FKHRL1 around the phosphorylation site of serine 253 (A-V-SP-M-D).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityFKHRL1 (phospho-Ser253) antibody detects endogenous levels of FKHRL1 only when phosphorylated at serine 253.
ReferencesSmith WW, et al. (2005) J Cell Biol; 169(2): 331-9.
Essafi A, et al. (2005) Oncogene; 24(14): 2317-29.
Brunet A, et al. (2001)Mol Cell Biol; 21(3): 952-65.
Brunet A, et al. (1999) Cell; 96(6): 857-68.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells