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Primary Antibodies

PAK1(Phospho-Thr212) Antibody

E011154-1Cite ID: EN0000299DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50-1:100
Q13153
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-TP-P-T).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityPAK1 (phospho-Thr212) antibody detects endogenous levels of PAK1 only when phosphorylated at threonine 212.
ReferencesAlexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819
Thiel DA, et al. (2002) Curr Biol; 12:1227-1232
Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 - 49052.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1 (phospho-Thr212) antibody (E011154).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1 (phospho-Thr212) antibody (E011154).
Western blot analysis of extracts from 293 cells, untreated or treated with forskolin (40μM, 30min), using PAK1 (Ab-212) antibody (E021160, Lane 1 and 2) and PAK1 (phospho-Thr212) antibody (E011154, Lane 3 and 4).
Western blot analysis of extracts from 293 cells, untreated or treated with forskolin (40μM, 30min), using PAK1 (Ab-212) antibody (E021160, Lane 1 and 2) and PAK1 (phospho-Thr212) antibody (E011154, Lane 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011154-1-Conjugate. Price: $485 / Size: 100μl.

E011154-1-FITC$485 / 100μlE011154-1-Biotin$485 / 100μlE011154-1-HRP$485 / 100μlE011154-1-AP$485 / 100μlE011154-1-AF350$485 / 100μlE011154-1-AF405M$485 / 100μlE011154-1-AF488$485 / 100μlE011154-1-AF514$485 / 100μlE011154-1-AF532$485 / 100μlE011154-1-AF555$485 / 100μlE011154-1-AF568$485 / 100μlE011154-1-AF405S$485 / 100μlE011154-1-AF405L$485 / 100μlE011154-1-AF546$485 / 100μlE011154-1-AF594$485 / 100μlE011154-1-AF610$485 / 100μlE011154-1-AF635$485 / 100μlE011154-1-AF647$485 / 100μlE011154-1-AF680$485 / 100μlE011154-1-AF700$485 / 100μlE011154-1-AF750$485 / 100μlE011154-1-AF790$485 / 100μlE011154-1-APC$485 / 100μlE011154-1-PE$485 / 100μlE011154-1-Cy3$485 / 100μlE011154-1-Cy5$485 / 100μlE011154-1-Cy5.5$485 / 100μlE011154-1-Cy7$485 / 100μlE011154-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.