Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human IκB-α around the phosphorylation site of serine 32/36 (H-D-SP-G-L-D-SP -M-K).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityIκB-α (phospho-Ser32/Ser36) antibody detects endogenous levels of IκB-α only when phosphorylated at serine 32/36.
ReferencesShieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823.
Honda, R. et al. (1997) FEBS Lett. 420, 25-27.
Hirao, A. et al. (2000) Science 287, 1824-1827.
Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157.
Chehab,N.H.et al. (1999) Proc .Natl .Acad. Sci. USA 96, 13777-13782.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells