Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Vav around the phosphorylation site of tyrosine 174 (E-I-YP-E-D).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityVav (phospho-Tyr174) antibody detects endogenous levels of Vav only when phosphorylated at tyrosine 174.
ReferencesAmarasinghe GK, et al. (2005) Biochemistry; 44(46): 15257-68. Charvet C, et al. (2005) J Biol Chem; 280(15): 15289-99. Miura-Shimura Y, et al. (2003) J Biol Chem; 278(40): 38495-504. Kuhne MR, et al. (2000) J Biol Chem; 275(3): 2185-90.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells