All Names
AlternameCHEK1, Chk1, kinase Chk1
All SitesHuman: Ser345; Mouse: Ser345; Rat: Ser345
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 345 (S-F-SP-Q-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityChk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.
ReferencesShiromizu T, et al. (2006) Genes CellsMay: 11(5): 477-85.
Hang YW, et al. (2005) Mol Cell: 19(5): 607-18.
Lu X, et al. (2005) Genes Dev: 19(10): 1162-74.
Falck J, et al. (2005) Nature: 434(7033): 605-11.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells