Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of threonine 231 (V-R-TP-P-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityTau (phospho-Thr231) antibody detects endogenous levels of Tau only when phosphorylated at threonine 231.
ReferencesYen S.H.et.al. (1997) Neurochem. 69:1709-1719.
Beausoleil S.A.et.al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135.
Wintjens R.et.al. (2001) Biol. Chem. 276:25150-25156.
Babu J.R.et.al.(2005) Neurochem. 94:192-203.
Goedert M., Spillantini M.G. (2000) Biochim. Biophys. Acta 1502:110-121.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells