Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 315 (S-S-SP-P-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivityp53 (phospho-Ser315) antibody detects endogenous levels of p53 only when phosphorylated at serine 315.
ReferencesLu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.
Lohrum, M. et.al. (1996) Oncogene 13, 2527-2539.
Ulrich, S. J. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 5954-5958.
Pospísilová S, et al. (2004) Biochem J; 378(Pt 3): 939-47.
Merrick BA, et al. (2001) Biochemistry; 40(13): 4053-66.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells