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Primary Antibodies

p53 (Phospho-Ser315) Antibody

E011100-1Cite ID: EN0000195DatasheetCOAMSDS
Human
WB: 1:500~1:1000 IHC: 1:50~1:100
P04637
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 315 (S-S-SP-P-Q).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivityp53 (phospho-Ser315) antibody detects endogenous levels of p53 only when phosphorylated at serine 315.
ReferencesLu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.
Lohrum, M. et.al. (1996) Oncogene 13, 2527-2539.
Ulrich, S. J. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 5954-5958.
Pospísilová S, et al. (2004) Biochem J; 378(Pt 3): 939-47.
Merrick BA, et al. (2001) Biochemistry; 40(13): 4053-66.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (phospho- Ser315) antibody (E011100).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (phospho- Ser315) antibody (E011100).
Western blot analysis of extracts from ovary cancer cells using p53 (phosphor-Ser315) antibody (E011100).
Western blot analysis of extracts from ovary cancer cells using p53 (phosphor-Ser315) antibody (E011100).

Conjugated Antibody

This product is also available conjugated. Format: E011100-1-Conjugate. Price: $485 / Size: 100μl.

E011100-1-FITC$485 / 100μlE011100-1-Biotin$485 / 100μlE011100-1-HRP$485 / 100μlE011100-1-AP$485 / 100μlE011100-1-AF350$485 / 100μlE011100-1-AF405M$485 / 100μlE011100-1-AF488$485 / 100μlE011100-1-AF514$485 / 100μlE011100-1-AF532$485 / 100μlE011100-1-AF555$485 / 100μlE011100-1-AF568$485 / 100μlE011100-1-AF405S$485 / 100μlE011100-1-AF405L$485 / 100μlE011100-1-AF546$485 / 100μlE011100-1-AF594$485 / 100μlE011100-1-AF610$485 / 100μlE011100-1-AF635$485 / 100μlE011100-1-AF647$485 / 100μlE011100-1-AF680$485 / 100μlE011100-1-AF700$485 / 100μlE011100-1-AF750$485 / 100μlE011100-1-AF790$485 / 100μlE011100-1-APC$485 / 100μlE011100-1-PE$485 / 100μlE011100-1-Cy3$485 / 100μlE011100-1-Cy5$485 / 100μlE011100-1-Cy5.5$485 / 100μlE011100-1-Cy7$485 / 100μlE011100-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.