Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 37 (L-P-SP-Q-A).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivityp53 (phospho-Ser37) antibody detects endogenous levels of p53 only when phosphorylated at serine 37.
ReferencesIto, A. et al. (2001) EMBO J. 20, 1331-1340.
Sakaguchi, K. et al. (1998) Genes Dev. 12, 2831-2841.
Solomon, J.M. et al. (2006) Mol. Cell. Biol. 26, 28-38.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells