World supplier of antibodies, antigens and kits since 2007
Primary Antibodies

p53 (Phospho-Thr18) Antibody

E011095-1Cite ID: EN0000185DatasheetCOAMSDS
Human
WB: 1:500~1:1000
P04637
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of threonine 18 (Q-E-TP-F-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivityp53 (Phospho-Thr18) antibody detects endogenous levels of p53 only when phosphorylated at threonine 18.
ReferencesLin T, et al. (2005) Nat Cell Biol; 7(2): 165-71.
Vega FM, et al. (2004) Mol Cell Biol; 24(23): 10366-80.
Li J, et al. (2004) J Biol Chem; 279(40): 41275-9.
Wang J, et al. (2004) J Biol Chem; 279(38): 39584-92.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from HT-29 cells untreated or treated with hydroxyurea, using p53 (Ab-18) antibody (E021086, Lane 1 and 2) and p53 (phospho-Thr18) antibody (E011095, Lane 3 and 4).
Western blot analysis of extracts from HT-29 cells untreated or treated with hydroxyurea, using p53 (Ab-18) antibody (E021086, Lane 1 and 2) and p53 (phospho-Thr18) antibody (E011095, Lane 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011095-1-Conjugate. Price: $485 / Size: 100μl.

E011095-1-FITC$485 / 100μlE011095-1-Biotin$485 / 100μlE011095-1-HRP$485 / 100μlE011095-1-AP$485 / 100μlE011095-1-AF350$485 / 100μlE011095-1-AF405M$485 / 100μlE011095-1-AF488$485 / 100μlE011095-1-AF514$485 / 100μlE011095-1-AF532$485 / 100μlE011095-1-AF555$485 / 100μlE011095-1-AF568$485 / 100μlE011095-1-AF405S$485 / 100μlE011095-1-AF405L$485 / 100μlE011095-1-AF546$485 / 100μlE011095-1-AF594$485 / 100μlE011095-1-AF610$485 / 100μlE011095-1-AF635$485 / 100μlE011095-1-AF647$485 / 100μlE011095-1-AF680$485 / 100μlE011095-1-AF700$485 / 100μlE011095-1-AF750$485 / 100μlE011095-1-AF790$485 / 100μlE011095-1-APC$485 / 100μlE011095-1-PE$485 / 100μlE011095-1-Cy3$485 / 100μlE011095-1-Cy5$485 / 100μlE011095-1-Cy5.5$485 / 100μlE011095-1-Cy7$485 / 100μlE011095-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.