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Primary Antibodies

p53 (Phospho-Ser15) Antibody

E011094-1Cite ID: EN0000183DatasheetCOAMSDS
Human
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200
P04637
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 15 (P-L-SP-Q-E).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivityp53 (phospho-Ser15) antibody detects endogenous levels of p53 only when phosphorylated at serine15.
ReferencesShieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823.
Honda, R. et al. (1997) FEBS Lett. 420, 25-27.
Hirao, A. et al. (2000) Science 287, 1824-1827.
Price145$

All Names

AlternameA-T, mutated, Ataxia telangiectasia mutated, Ataxia telangiectasia mutated homolog, Serine-protein kinase ATM, kinase ATM
All SitesHuman: Ser1981; Mouse: Ser1987
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (phospho- Ser15) antibody (E011094).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (phospho- Ser15) antibody (E011094).
Western blot analysis of the extracts from HeLa cells untreated or treated with hydroxyurea, using p53 (Ab-15) antibody (E021085, Line1 and 2) and p53 (phospho-Ser15) antibody (E011094, Line3 and 4).
Western blot analysis of the extracts from HeLa cells untreated or treated with hydroxyurea, using p53 (Ab-15) antibody (E021085, Line1 and 2) and p53 (phospho-Ser15) antibody (E011094, Line3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011094-1-Conjugate. Price: $485 / Size: 100μl.

E011094-1-FITC$485 / 100μlE011094-1-Biotin$485 / 100μlE011094-1-HRP$485 / 100μlE011094-1-AP$485 / 100μlE011094-1-AF350$485 / 100μlE011094-1-AF405M$485 / 100μlE011094-1-AF488$485 / 100μlE011094-1-AF514$485 / 100μlE011094-1-AF532$485 / 100μlE011094-1-AF555$485 / 100μlE011094-1-AF568$485 / 100μlE011094-1-AF405S$485 / 100μlE011094-1-AF405L$485 / 100μlE011094-1-AF546$485 / 100μlE011094-1-AF594$485 / 100μlE011094-1-AF610$485 / 100μlE011094-1-AF635$485 / 100μlE011094-1-AF647$485 / 100μlE011094-1-AF680$485 / 100μlE011094-1-AF700$485 / 100μlE011094-1-AF750$485 / 100μlE011094-1-AF790$485 / 100μlE011094-1-APC$485 / 100μlE011094-1-PE$485 / 100μlE011094-1-Cy3$485 / 100μlE011094-1-Cy5$485 / 100μlE011094-1-Cy5.5$485 / 100μlE011094-1-Cy7$485 / 100μlE011094-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

p53 (Phospho-Ser15) Antibody | EnoGene Biotech Co. Ltd