Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine1248 (P-E-YP-L-G).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityHER2 (phospho-Tyr1248) antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine1248.
ReferencesRamsauer VP, et al. (2003) J Biol Chem; 278(32): 30142-7.
Jepson S, et al. (2002) Oncogene; 21(49): 7524-32.
Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.
Eppenberger-Castori S, et al. (2001) J Clin Oncol; 19(3): 645-56.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells