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Primary Antibodies

HER2 (Phospho-Tyr1221/Tyr1222) Antibody

E011076-1Cite ID: EN0000149DatasheetCOAMSDS
Human
WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
P04626
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine1221/1222 (N-L-YP-YP -W).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityHER2 (phospho-Tyr1221/Tyr1222) antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine 1221/1222.
ReferencesMarone R, et al. (2004) Nat Cell Biol; 6(6): 515-22.
Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
paraffin- embedded human Immunofluorescence staining of carcinoma tissue using methanol-fixed MCF7 cells using (phospho-Tyr1221/Tyr1222) HER2 (phospho- Tyr1221/ Tyr1222) (E011076). antibody (E011076, Red).
paraffin- embedded human Immunofluorescence staining of carcinoma tissue using methanol-fixed MCF7 cells using (phospho-Tyr1221/Tyr1222) HER2 (phospho- Tyr1221/ Tyr1222) (E011076). antibody (E011076, Red).
Immunohistochemical analysis paraffin- embedded human Immunofluorescence staining of carcinoma tissue using methanol-fixed MCF7 cells using (phospho-Tyr1221/Tyr1222) HER2 (phospho- Tyr1221/ Tyr1222) (E011076). antibody (E011076, Red).
Immunohistochemical analysis paraffin- embedded human Immunofluorescence staining of carcinoma tissue using methanol-fixed MCF7 cells using (phospho-Tyr1221/Tyr1222) HER2 (phospho- Tyr1221/ Tyr1222) (E011076). antibody (E011076, Red).
HER2 Western blot analysis of extracts from SK-OV3 cells antibody using HER2 (Ab-1221/1222) antibody (E021071, Line 1 and 2) and HER2 (phospho-Tyr1221/Tyr1222) antibody (E011076, Line 3 and 4).
HER2 Western blot analysis of extracts from SK-OV3 cells antibody using HER2 (Ab-1221/1222) antibody (E021071, Line 1 and 2) and HER2 (phospho-Tyr1221/Tyr1222) antibody (E011076, Line 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011076-1-Conjugate. Price: $485 / Size: 100μl.

E011076-1-FITC$485 / 100μlE011076-1-Biotin$485 / 100μlE011076-1-HRP$485 / 100μlE011076-1-AP$485 / 100μlE011076-1-AF350$485 / 100μlE011076-1-AF405M$485 / 100μlE011076-1-AF488$485 / 100μlE011076-1-AF514$485 / 100μlE011076-1-AF532$485 / 100μlE011076-1-AF555$485 / 100μlE011076-1-AF568$485 / 100μlE011076-1-AF405S$485 / 100μlE011076-1-AF405L$485 / 100μlE011076-1-AF546$485 / 100μlE011076-1-AF594$485 / 100μlE011076-1-AF610$485 / 100μlE011076-1-AF635$485 / 100μlE011076-1-AF647$485 / 100μlE011076-1-AF680$485 / 100μlE011076-1-AF700$485 / 100μlE011076-1-AF750$485 / 100μlE011076-1-AF790$485 / 100μlE011076-1-APC$485 / 100μlE011076-1-PE$485 / 100μlE011076-1-Cy3$485 / 100μlE011076-1-Cy5$485 / 100μlE011076-1-Cy5.5$485 / 100μlE011076-1-Cy7$485 / 100μlE011076-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.