Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine 877 (T-E-YP-H-A).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorrylation site.
Specificty Sensitivity
Specificity/SensitivityHER2 (phospho-Tyr877) antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine 877.
ReferencesDittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444.
Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857.
Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells