Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Estrogen Receptor-α around the phosphorylation site of serine 167 (L-A-SP-T-N).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityEstrogen Receptor-α (phospho-Ser167) antibody detects endogenous levels of Estrogen Receptor-α only when phosphorylated at serine167.
ReferencesShah YM, et al. (2005). Mol Endocrinol.19 (3): 732-748.
Sun M, et al. (2001). Cancer Res.61 (16): 5985-5991.
Campbell RA, et al. (2001). J Biol Chem.276 (13): 9817-9824.
Chen D, et al. (2000). Mol Cell.6 1): 127-137.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells