Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Estrogen Receptor-α around the phosphorylation site of serine 118 (Q-L-SP-P-F).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityEstrogen Receptor-α (phospho-Ser118) antibody detects endogenous levels of Estrogen Receptor-α only when phosphorylated at serine 118.
ReferencesMedunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014.
Masuhiro Y, et al. (2005). Proc Natl Acad Sci U S A.102 (23): 8126-8131.
Pentecost BT, et al. (2005). Mol Cell Endocrinol.238 (1-2): 9-25.
Park KJ, et al. (2005). Mol Cell.18 (1): 71-82.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells