Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-SP-P-L).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityBCL-2 (phospho-Ser70) antibody detects endogenous levels of BCL-2 only when phos- phorylated at serine 70.
ReferencesJin Z, Gao F,et al. J Biol Chem 2004 Sep 17; 279(38): 40209-19.
Kumar Biswas S, et al. Mol Cancer Ther 2004 Mar; 3(3): 327-34.
Huang ST, et al. (2002) FASEB J; 16(8): 825-32.
Yamamoto K, et al. (1999) Mol Cell Biol; 19(12): 8469-78.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells