Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 56 (G-H-TP-P-H).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityBCL-2 (phospho-Thr56) antibody detects endogenous levels of BCL-2 only when phosphorylated at threonine 56.
ReferencesLing, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991.
Huang, S.J. and Cidlowski, J.A. (2002) FASEB 16, 825-832.
Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688.
Huang ST,et al. (2002) FASEB J Jun; 16(8): 825-32.
Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469-8478.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells