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Primary Antibodies

Ezrin(Phospho-Tyr353) Antibody

E011063-1Cite ID: EN0000123DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 IHC: 1:50~1:100
P15311
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameCytovillin, EZRI, VIL2, Villin 2, p81
All SitesHuman: Tyr353; Mouse: Tyr353

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 353 (Q-D-YP-E-E).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityEzrin (phospho-Tyr353) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 353.
ReferencesZhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-9490.
Wang Q, et al. (2003) J Biol Chem 278(48): 47731-47743.
Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96(13): 7300-7305.
Crepaldi T, et al. (1997) J Cell Biol 138(2): 423-34.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Ezrin (phospho- Tyr353) antibody (E011063).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Ezrin (phospho- Tyr353) antibody (E011063).
Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min), using Ezrin (Ab-353) antibody (E021094, Lane 1 and 2) and Ezrin (phospho-Tyr353) antibody (E011063, Lane 3 and 4).
Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min), using Ezrin (Ab-353) antibody (E021094, Lane 1 and 2) and Ezrin (phospho-Tyr353) antibody (E011063, Lane 3 and 4).

Conjugated Antibody

This product is also available conjugated. Format: E011063-1-Conjugate. Price: $485 / Size: 100μl.

E011063-1-FITC$485 / 100μlE011063-1-Biotin$485 / 100μlE011063-1-HRP$485 / 100μlE011063-1-AP$485 / 100μlE011063-1-AF350$485 / 100μlE011063-1-AF405M$485 / 100μlE011063-1-AF488$485 / 100μlE011063-1-AF514$485 / 100μlE011063-1-AF532$485 / 100μlE011063-1-AF555$485 / 100μlE011063-1-AF568$485 / 100μlE011063-1-AF405S$485 / 100μlE011063-1-AF405L$485 / 100μlE011063-1-AF546$485 / 100μlE011063-1-AF594$485 / 100μlE011063-1-AF610$485 / 100μlE011063-1-AF635$485 / 100μlE011063-1-AF647$485 / 100μlE011063-1-AF680$485 / 100μlE011063-1-AF700$485 / 100μlE011063-1-AF750$485 / 100μlE011063-1-AF790$485 / 100μlE011063-1-APC$485 / 100μlE011063-1-PE$485 / 100μlE011063-1-Cy3$485 / 100μlE011063-1-Cy5$485 / 100μlE011063-1-Cy5.5$485 / 100μlE011063-1-Cy7$485 / 100μlE011063-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

Ezrin(Phospho-Tyr353) Antibody | EnoGene Biotech Co. Ltd