Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 68 (V-S-TP-Q-E).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityChk2 (phospho-Thr68) antibody detects endogenous levels of Chk2 only when phosphorylated at threonine 68.
ReferencesGorgoulis VG, et al. (2005) Nature; 434(7035): 907-13.
Falck J, et al. (2005) Nature; 434(7033): 605-11.
Jin ZH, et al. (2005) Oncogene; 24(12): 1973-81.
Li J, et al. (2005) J Biol Chem; 280(12): 12041-50.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells