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Primary Antibodies

PTEN (Phospho-Ser380/Thr382/Thr383) Antibody

E011056-1Cite ID: EN0000109DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC:1:50~100 IF:1:100~1:200
P60484
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human PTEN around the phosphorylation site of serine 380 and threonine 382/383 (R-Y-SP-D-TP-TP-D-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityPTEN (phospho-Ser380/Thr382/Thr383) antibody detects endogenous levels of PTEN only when phosphorylated at serine 380 and threonine 382/383.
ReferencesAl-Khouri AM, et al. (2005) J Biol Chem. 280(42):35195-35202.
Torres J, et al. (2001) J Biol Chem. 276(2): 993-998.
Vazquez F, et al. (2000) Mol Cell Biol. 20(14): 5010-5018.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using PTEN (phospho-Ser380/Thr382/Thr383) antibody (E011056).
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using PTEN (phospho-Ser380/Thr382/Thr383) antibody (E011056).
Western blot analysis of extract from HT29 cells untreated or treated with calyculin A, using PTEN (phospho-Ser380/Thr382/Thr383) antibody (E011056).
Western blot analysis of extract from HT29 cells untreated or treated with calyculin A, using PTEN (phospho-Ser380/Thr382/Thr383) antibody (E011056).

Conjugated Antibody

This product is also available conjugated. Format: E011056-1-Conjugate. Price: $485 / Size: 100μl.

E011056-1-FITC$485 / 100μlE011056-1-Biotin$485 / 100μlE011056-1-HRP$485 / 100μlE011056-1-AP$485 / 100μlE011056-1-AF350$485 / 100μlE011056-1-AF405M$485 / 100μlE011056-1-AF488$485 / 100μlE011056-1-AF514$485 / 100μlE011056-1-AF532$485 / 100μlE011056-1-AF555$485 / 100μlE011056-1-AF568$485 / 100μlE011056-1-AF405S$485 / 100μlE011056-1-AF405L$485 / 100μlE011056-1-AF546$485 / 100μlE011056-1-AF594$485 / 100μlE011056-1-AF610$485 / 100μlE011056-1-AF635$485 / 100μlE011056-1-AF647$485 / 100μlE011056-1-AF680$485 / 100μlE011056-1-AF700$485 / 100μlE011056-1-AF750$485 / 100μlE011056-1-AF790$485 / 100μlE011056-1-APC$485 / 100μlE011056-1-PE$485 / 100μlE011056-1-Cy3$485 / 100μlE011056-1-Cy5$485 / 100μlE011056-1-Cy5.5$485 / 100μlE011056-1-Cy7$485 / 100μlE011056-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.