Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-TP-F-C).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityAkt (phospho-Thr308) antibody detects endogenous levels of Akt only when phosphorylated at threonine 308.
ReferencesTremblay F, et al. (2005)Diabetes; 54(9): 2674-84.
Xu BE, et al. (2005)J Biol Chem; 280(40): 34218-23.
Samuels Y, et al. (2005)Cancer Cell; 7(6): 561-73.
Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells