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Primary Antibodies

Akt (Phospho-Thr308) Antibody

E011055-1Cite ID: EN0000107DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200
P31749
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-TP-F-C).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityAkt (phospho-Thr308) antibody detects endogenous levels of Akt only when phosphorylated at threonine 308.
ReferencesTremblay F, et al. (2005)Diabetes; 54(9): 2674-84.
Xu BE, et al. (2005)J Biol Chem; 280(40): 34218-23.
Samuels Y, et al. (2005)Cancer Cell; 7(6): 561-73.
Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (phospho-Thr308) antibody(E011055).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (phospho-Thr308) antibody(E011055).
Western blot analysis using Akt (phospho-Thr308) antibody (E011055): Lane1: The extract from 293 cells untreated; Lane 2: The extract from 293 cells treated with insulin.
Western blot analysis using Akt (phospho-Thr308) antibody (E011055): Lane1: The extract from 293 cells untreated; Lane 2: The extract from 293 cells treated with insulin.

Conjugated Antibody

This product is also available conjugated. Format: E011055-1-Conjugate. Price: $485 / Size: 100μl.

E011055-1-FITC$485 / 100μlE011055-1-Biotin$485 / 100μlE011055-1-HRP$485 / 100μlE011055-1-AP$485 / 100μlE011055-1-AF350$485 / 100μlE011055-1-AF405M$485 / 100μlE011055-1-AF488$485 / 100μlE011055-1-AF514$485 / 100μlE011055-1-AF532$485 / 100μlE011055-1-AF555$485 / 100μlE011055-1-AF568$485 / 100μlE011055-1-AF405S$485 / 100μlE011055-1-AF405L$485 / 100μlE011055-1-AF546$485 / 100μlE011055-1-AF594$485 / 100μlE011055-1-AF610$485 / 100μlE011055-1-AF635$485 / 100μlE011055-1-AF647$485 / 100μlE011055-1-AF680$485 / 100μlE011055-1-AF700$485 / 100μlE011055-1-AF750$485 / 100μlE011055-1-AF790$485 / 100μlE011055-1-APC$485 / 100μlE011055-1-PE$485 / 100μlE011055-1-Cy3$485 / 100μlE011055-1-Cy5$485 / 100μlE011055-1-Cy5.5$485 / 100μlE011055-1-Cy7$485 / 100μlE011055-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.