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Primary Antibodies

Akt (Phospho-Ser473) Antibody

E011054-1Cite ID: EN0000105DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100
P31749
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityAkt (phospho-Ser473) antibody detects endogenous levels of Akt only when phosphorylated at serine 473.
ReferencesBaudhuin LM, et al. (2004) FASEB J Feb; 18(2): 341-3.
Min YH, et al. (2004) Cancer Res; 64(15): 5225-31.
Feng J, et al. (2004) J Biol Chem; 279(34): 35510-7.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using Akt (phospho-Ser473) antibody (E011054).
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using Akt (phospho-Ser473) antibody (E011054).
Western blot analysis of extract from HeLa cells untreated or treated with heat shock using Akt (Ab-473) antibody (E021054, Lane 1 and 2) and Akt (phospho-Ser473) antibody (E011054, Lane 3 and 4).
Western blot analysis of extract from HeLa cells untreated or treated with heat shock using Akt (Ab-473) antibody (E021054, Lane 1 and 2) and Akt (phospho-Ser473) antibody (E011054, Lane 3 and 4).
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E011054-1-Conjugate. Price: $485 / Size: 100μl.

E011054-1-FITC$485 / 100μlE011054-1-Biotin$485 / 100μlE011054-1-HRP$485 / 100μlE011054-1-AP$485 / 100μlE011054-1-AF350$485 / 100μlE011054-1-AF405M$485 / 100μlE011054-1-AF488$485 / 100μlE011054-1-AF514$485 / 100μlE011054-1-AF532$485 / 100μlE011054-1-AF555$485 / 100μlE011054-1-AF568$485 / 100μlE011054-1-AF405S$485 / 100μlE011054-1-AF405L$485 / 100μlE011054-1-AF546$485 / 100μlE011054-1-AF594$485 / 100μlE011054-1-AF610$485 / 100μlE011054-1-AF635$485 / 100μlE011054-1-AF647$485 / 100μlE011054-1-AF680$485 / 100μlE011054-1-AF700$485 / 100μlE011054-1-AF750$485 / 100μlE011054-1-AF790$485 / 100μlE011054-1-APC$485 / 100μlE011054-1-PE$485 / 100μlE011054-1-Cy3$485 / 100μlE011054-1-Cy5$485 / 100μlE011054-1-Cy5.5$485 / 100μlE011054-1-Cy7$485 / 100μlE011054-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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Akt (Phospho-Ser473) Antibody | EnoGene Biotech Co. Ltd