Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human CREB around the phosphorylation site of serine 133 (R-P-SP-Y-R).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityCREB (phospho-Ser133) antibody detects endogenous levels of CREB only when phosphorylated at serine 133.
ReferencesXing J, et al. (1998) Mol Cell Biol 18(4): 1946-55.
Tan Y, et al. (1996) EMBO J; 15(17): 4629-42.
Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.
Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells