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Primary Antibodies

NFκB-p100/p52 (Ser869) Antibody

E011016-1Cite ID: EN0000030DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
Q00653
$145/ 50μg/50μl
Specification
Quantity
Total: $145

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human NF-κB around the phosphorylation site of serine 869 (Y-G-SP-Q-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityNF-κB p100/p52 (phospho-Ser869) antibody detects endogenous levels of NF-κB p100/p52 only when phosphorylated at serine 869.
ReferencesQu Z, et al. (2004) J Biol Chem; 279(43): 44563-72.
Xiao G, et al. (2001) J Biol Chem 7(2): 401-9.
Price145$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p100/p52 (phospho- Ser869) antibody (E011016).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p100/p52 (phospho- Ser869) antibody (E011016).
Western blot analysis of extract from MDA-MB-435 cells untreated or treated with TNF-α (20ng/ml, 5min) using NF-κB p100/p52 (phospho-Ser869) antibody (E011016).
Western blot analysis of extract from MDA-MB-435 cells untreated or treated with TNF-α (20ng/ml, 5min) using NF-κB p100/p52 (phospho-Ser869) antibody (E011016).

Conjugated Antibody

This product is also available conjugated. Format: E011016-1-Conjugate. Price: $485 / Size: 100μl.

E011016-1-FITC$485 / 100μlE011016-1-Biotin$485 / 100μlE011016-1-HRP$485 / 100μlE011016-1-AP$485 / 100μlE011016-1-AF350$485 / 100μlE011016-1-AF405M$485 / 100μlE011016-1-AF488$485 / 100μlE011016-1-AF514$485 / 100μlE011016-1-AF532$485 / 100μlE011016-1-AF555$485 / 100μlE011016-1-AF568$485 / 100μlE011016-1-AF405S$485 / 100μlE011016-1-AF405L$485 / 100μlE011016-1-AF546$485 / 100μlE011016-1-AF594$485 / 100μlE011016-1-AF610$485 / 100μlE011016-1-AF635$485 / 100μlE011016-1-AF647$485 / 100μlE011016-1-AF680$485 / 100μlE011016-1-AF700$485 / 100μlE011016-1-AF750$485 / 100μlE011016-1-AF790$485 / 100μlE011016-1-APC$485 / 100μlE011016-1-PE$485 / 100μlE011016-1-Cy3$485 / 100μlE011016-1-Cy5$485 / 100μlE011016-1-Cy5.5$485 / 100μlE011016-1-Cy7$485 / 100μlE011016-1-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.