Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human NF-κB around the phosphorylation site of serine 869 (Y-G-SP-Q-S).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityNF-κB p100/p52 (phospho-Ser869) antibody detects endogenous levels of NF-κB p100/p52 only when phosphorylated at serine 869.
ReferencesQu Z, et al. (2004) J Biol Chem; 279(43): 44563-72.
Xiao G, et al. (2001) J Biol Chem 7(2): 401-9.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells