Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human NF-κB p100/p52 around the phosphorylation site of serine 865 (E-D-SP-A-Y).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityNF-κB p100/p52 (phospho-ser865) antibody detects endogenous levels of NF-κB p100/p52 only when phosphorylated at serine 865.
ReferencesQu Z, et al. (2004) J Biol Chem. 279(43): 44563-44572.
Xiao G, et al. (2001) Mol Cell. 7(2): 401-409.
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.
Baeuerle P A, et al. (1996) Cell 87:13-20.