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PKC-pan (Phospho-Thr497) Antibody
Datasheet   

  • Catalog Number:

    E11-0803A

  • Amount:

    100μg

  • Swiss-Prot No.:

    P17252/P05771/Q05655/Q02156/ P05129/P24723/Q04759/Q05513

  • Other Names:

    EC 2.7.11.13, KPCA, PKC III, PKC-A, PKC-III, PKC-alpha, PRKACA, PRKCA, Protein kinase C, alpha type, kinase PKC-alpha; PKC-delta, PRKCD, kinase PKC-delta, nPKC-delta; PKC-epsilon, PKCEA, PRKCE, epsilon type, kinase PKC-epsilon, nPKC-epsilon

  • All Sites:

    Human: Thr497; Mouse: Thr497; Rat: Thr497

  • Storage/Stability:

    Store at -20℃/1 year

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human PKC-pan around the phosphorylation site of threonine 497 (T-S-TP-F-C).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    PKC-pan (Phospho-Thr497) antibody detects endogenous levels of PKC-pan only when phosphorylated at threonine 497.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

  • References:

    Gevaert K., Nat. Biotechnol. 21:566-569(2003).
    Wissing J., Mol. Cell. Proteomics 6:537-547(2007).
    Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
    Hillier L.W., Nature 434:724-731(2005).

  • P-peptide - +

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC-pan (Phospho-Thr497) antibody.
    Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins), using PKC-pan (Phospho-Thr497) antibody.

  • P-peptide - +
    Immunofluorescence analysis of HepG2 cells, using PKC-pan (Phospho-Thr497) antibody.

  • Price:

    225$

Add to Cart

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Catalog Number

  • Amount:100μg
  • Price:225$

Add to Cart

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PKC-pan (Phospho-Thr497) Antibody | EnoGene Biotech Co. Ltd