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Chk2 (Phospho-Thr383) Antibody
Datasheet
Catalog Number:
E11-0784AAmount:
100μgSwiss-Prot No.:
O96017Other Names:
CHEK2, CHK2, Cds1, Chk2, EC 2.7.11.1, RAD53, kinase Chk2All Sites:
Human: Thr383; Mouse: Thr387; Rat: Thr386Storage/Stability:
Store at -20℃/1 yearForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 383 (M-R-TP-L-C).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
Chk2 (Phospho-Thr383) antibody detects endogenous levels of Chk2 only when phosphorylated at threonine 383.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000References:
Shannon L. Gibson, Cancer Res., Dec 2005; 65: 10734 - 10741.
Jinwoo Ahn, J. Biol. Chem., Dec 2002; 277: 48418 - 48426.
Nabil H. Chehab, Genes & Dev., Feb 2000; 14: 278.
- P-peptide - +
Western blot analysis of extracts from COS7 cells, treated with UV (30mins), using Chk2 (Phospho-Thr383) antibody.
Immunofluorescence analysis of HeLa cells, using Chk2 (Phospho-Thr383) antibody. Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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