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B-RAF (Phospho-Thr599) Antibody
Datasheet
Catalog Number:
E11-0780AAmount:
100μgSwiss-Prot No.:
P15056Other Names:
B-RAF, B-RAF proto-oncogene serine/threonine-protein kinase, B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, EC 2.7.1.37, EC 2.7.11.1, RAFB1, RMIL, RMIL serine/threonine-protein kinase, c-RMIL, kinase B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1All Sites:
Human: Thr598; Mouse: Thr626; Rat: Thr636Storage/Stability:
Store at -20℃/1 yearForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human B-Raf around the phosphorylation site of threonine 598 (L-A-TP-V-K).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
B-Raf (Phospho-Thr598) antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598.Reactivity:
Human, Mouse, RatApplications:
IHC: 1:50~1:100 ELISA: 1:40000References:
Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 - 42418.
Tara J. Dillon, J. Biol. Chem., Sep 2003; 278: 35940 - 35949.
R K Jaiswal, Mol. Cell. Biol., Oct 1994; 14: 6944 - 6953.
Laura L. Dugan, J. Biol. Chem., Sep 1999; 274: 25842.
- P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using B-Raf (Phospho-Thr598) antibody. Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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