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Survivin (Phospho-Thr34) Antibody
Datasheet
Catalog Number:
E11-0578AAmount:
100μgSwiss-Prot No.:
O15392Other Names:
API4; apoptosis inhibitor 4; apoptosis inhibitor survivin; baculoviral IAP repeat-containing protein 5; BIRC5; IAP4; TIAPAll Sites:
Human: Thr34; Mouse: Thr34Storage/Stability:
Store at -20℃/1 yearForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Survivin around the phosphorylation site of threonine 34 (A-C-TP-P-E).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
Survivin (Phospho-Thr34) antibody detects endogenous levels of Survivin only when phosphorylated at threonine 34.Reactivity:
Human, MouseApplications:
WB: 1:500~1:1000 ELISA: 1:1000References:
Ambrosini G., Nat. Med. 3:917-921(1997).
Mahotka C., Cancer Res. 59:6097-6102(1999).
Uren A.G., Curr. Biol. 10:1319-1328(2000).
- Western blot analysis of extracts from HT-29 cells, treated with EGF (100μg/ml, 30mins), using Survivin (Phospho-Thr34) antibody.
Price:
225$
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Catalog Number
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