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Mouse anti-Human CD41, Biotin Conjugated mAb.
Datasheet   

  • Catalog Number:

    e16HP0411-100/500

  • Amount:

    100Tests

  • Form of Antibody:

    Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide

  • Storage/Stability:

    Store at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

  • Background:

    4AHIP8 reacts with a calcium-dependent complex of CD41/CD61 ( GPIIb/IIIa), 135/120 kDa, expressed on normal platelets and megakaryocytes. CD41 antigen acts as a receptor for fibrinogen, von Willebrand factor (vWf), fibrinectin and vitronectin and mediates platelet adhesion and aggregation. GM1CD41 antibody completely inhibits ADP, epinephrine and collagen-induced platelet activation and partially inhibits restocetin and thrombin-induced platelet activation.  It is useful in the morphological and physiological studies of platelets and megakaryocytes.* Application: Diagnosis of Glanzmann's Thrombasthenia and megakaryoblastic leukemias, deletion of alloantibodies, and anti-thrombotic therapy.

  • Isotype:

    Mouse IgG1

  • Clone Number:

    4AHIP8

  • Applications:

    FC FA IHC IF Block

  • Experimental Methods:

    1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
    2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
    3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
    4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
    6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.
    7.Repeat step 5.
    8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
    [PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
    [Cell fixation: 2% formaldehyde solution]

  • Notices:

    1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
    3.If the sample can not be timely analysis, please fixed;
    4.For research use only, not for diagnostic or therapeutic use.

  • References:

    1. Knapp, W.,et al. (1989) Leucocyte Typing IV : White Cell Differentiation Antigens, Oxford University Press, New York.
    2. Feng R et al. (1998) Bone Marrow Transplant 12:121.

  • Related products:

    E16HU0411 Mouse Anti-Human CD41，Purified mAb FC FA IHC IF Block
    E16HP0411 Mouse Anti-Human CD41, PE Conjugated mAb FC FA IHC IF Block
    E16HC0411 Mouse Anti-Human CD41，PE-Cy5 Conjugated mAb FC FA IHC IF Block
    E16HF0411 Mouse Anti-Human CD41, FITC Conjugated mAb FC FA IHC IF Block
    Profile of Human platelet analyzed analyzed by flow cytometry

  • Human platelet analyzed analyzed with Biotin CD41 mAb, followed by Sav-PE

  • Price:

    225$

Add to Cart

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