Golgi-Tracker Red Kit
Datasheet
Catalog Number:
ETF1043Amount:
Storage/Stability:
Golgi-Tracer Red
1 vial
-20℃, Minimize light exposure
Staining Buffer
1 bottle (12.5mL)
DMSO
1 vial (100 µL)
PROTOCOL
PREPARATION OF STOCK SOLUTIONS
Add 50 µL of DMSO into the vial of Golgi-Tracer Red and mix well to make Golgi-Tracer Red stock solution .
Note: Store the unused Golgi-Tracer Red stock solution at -20 °C in single use aliquots to avoid freeze thaw cycles.
PREPARATION OF WORKING SOLUTION
Add 10 µL of Golgi-Tracer Red stock solution into 990 µL of Staining Buffer, and mix well to make Golgi-Tracer Red working solution.
Optional: Add 10 µL of Hoechst 33342 (not provided) to 1 mL Golgi-Tracer Red working solution for nuclear stain. Observe under fluorescence microscope with DAPI filter set.Sample:
EXPERIMENTFollowing protocol should be used for the guidelines and can be modified as per requirements.
Plate and treat cells as desired.
Add 100 µL of Golgi-Tracer Red working solution directly into cell culture medium.
Incubate at room temperature or 37 °C for 15-30 minutes.
Remove the Golgi-Tracer Red working solution and wash once with DPBS or buffer of your choice.
Add 100 µL/well of Staining Buffer.
6. Observe under a fluorescence microscope with Cy3 filter set.
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