World supplier of antibodies, antigens and kits since 2007
Cell biology kits
Golgi-Tracker Red Kit
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Storage/StabilityGolgi-Tracer Red
1 vial
-20℃, Minimize light exposure
Staining Buffer
1 bottle (12.5mL)
DMSO
1 vial (100 µL)
PROTOCOL
PREPARATION OF STOCK SOLUTIONS
Add 50 µL of DMSO into the vial of Golgi-Tracer Red and mix well to make Golgi-Tracer Red stock solution .
Note: Store the unused Golgi-Tracer Red stock solution at -20 °C in single use aliquots to avoid freeze thaw cycles.
PREPARATION OF WORKING SOLUTION
Add 10 µL of Golgi-Tracer Red stock solution into 990 µL of Staining Buffer, and mix well to make Golgi-Tracer Red working solution.
Optional: Add 10 µL of Hoechst 33342 (not provided) to 1 mL Golgi-Tracer Red working solution for nuclear stain. Observe under fluorescence microscope with DAPI filter set.
1 vial
-20℃, Minimize light exposure
Staining Buffer
1 bottle (12.5mL)
DMSO
1 vial (100 µL)
PROTOCOL
PREPARATION OF STOCK SOLUTIONS
Add 50 µL of DMSO into the vial of Golgi-Tracer Red and mix well to make Golgi-Tracer Red stock solution .
Note: Store the unused Golgi-Tracer Red stock solution at -20 °C in single use aliquots to avoid freeze thaw cycles.
PREPARATION OF WORKING SOLUTION
Add 10 µL of Golgi-Tracer Red stock solution into 990 µL of Staining Buffer, and mix well to make Golgi-Tracer Red working solution.
Optional: Add 10 µL of Hoechst 33342 (not provided) to 1 mL Golgi-Tracer Red working solution for nuclear stain. Observe under fluorescence microscope with DAPI filter set.
Product Information
SampleEXPERIMENTFollowing protocol should be used for the guidelines and can be modified as per requirements.
Plate and treat cells as desired.
Add 100 µL of Golgi-Tracer Red working solution directly into cell culture medium.
Incubate at room temperature or 37 °C for 15-30 minutes.
Remove the Golgi-Tracer Red working solution and wash once with DPBS or buffer of your choice.
Add 100 µL/well of Staining Buffer.
6. Observe under a fluorescence microscope with Cy3 filter set.
Plate and treat cells as desired.
Add 100 µL of Golgi-Tracer Red working solution directly into cell culture medium.
Incubate at room temperature or 37 °C for 15-30 minutes.
Remove the Golgi-Tracer Red working solution and wash once with DPBS or buffer of your choice.
Add 100 µL/well of Staining Buffer.
6. Observe under a fluorescence microscope with Cy3 filter set.
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