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Cell biology kits
ER-Tracker Red Kit
Price on request
Quantity
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Storage/StabilityER-Tracer-Red
1 vial
-20℃, Minimize light exposure
Staining Buffer
1 bottle (10 mL)
DMSO
1 vial (50 µL)
PROTOCOL
PREPARATION OF STOCK SOLUTIONS
ER-Tracer Red stock solution: Add 20 µL of DMSO into the vial of ER-Tracer Red and mix well to make ER-Tracer Red stock solution .
Note: Unused ER-Tracer Red stock solution can be stored at ≤ -20℃ for two weeks if the tubes are sealed tightly. Protect from light.
PREPARATION OF WORKING SOLUTION
ER-Tracer Red working solution: Add 20µL of ER-Tracer Red stock solution into 5mL of Staining Buffer, and mix well to make ER-Tracer Red working solution.
Note: This ER-Tracer Red working solution is stable for at least 2 hours at room temperature. Protect from light.
1 vial
-20℃, Minimize light exposure
Staining Buffer
1 bottle (10 mL)
DMSO
1 vial (50 µL)
PROTOCOL
PREPARATION OF STOCK SOLUTIONS
ER-Tracer Red stock solution: Add 20 µL of DMSO into the vial of ER-Tracer Red and mix well to make ER-Tracer Red stock solution .
Note: Unused ER-Tracer Red stock solution can be stored at ≤ -20℃ for two weeks if the tubes are sealed tightly. Protect from light.
PREPARATION OF WORKING SOLUTION
ER-Tracer Red working solution: Add 20µL of ER-Tracer Red stock solution into 5mL of Staining Buffer, and mix well to make ER-Tracer Red working solution.
Note: This ER-Tracer Red working solution is stable for at least 2 hours at room temperature. Protect from light.
Product Information
SampleEXPERIMENTAdd 100 µL/well (96-well plate) or 50 µL/well (384-well plate) of ER-Tracer Red working solution in the cell plate. Incubate cells with working solution at 37℃ for 15 - 30 minutes, protected from light.
Note: The optimal concentration of the ER probe varies depending on the specific application. Concentration higher than the working solution can be toxic to cells. The staining conditions may be modified according to the particular cell type and the permeability of the cells or tissues to the probe.
Remove ER-Tracer Red working solution in each well. Wash cells with physically relevant buffer three times.
Fix cells after staining (Optional). Fix the cells with 4% formaldehyde for 5-10 minutes. Wash cells with physically relevant buffer three times.
4. Observe the fluorescence signal in cells using fluorescence microscope with TRITC or Cy3 filter set (Ex/Em = 590/620 nm).
Note: The optimal concentration of the ER probe varies depending on the specific application. Concentration higher than the working solution can be toxic to cells. The staining conditions may be modified according to the particular cell type and the permeability of the cells or tissues to the probe.
Remove ER-Tracer Red working solution in each well. Wash cells with physically relevant buffer three times.
Fix cells after staining (Optional). Fix the cells with 4% formaldehyde for 5-10 minutes. Wash cells with physically relevant buffer three times.
4. Observe the fluorescence signal in cells using fluorescence microscope with TRITC or Cy3 filter set (Ex/Em = 590/620 nm).
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