Product Information
Cite ID#EN5550429
Expression systemSNRNP70,produced in E.coli is a single non-glycosylated polypeptide chain containing 210 amino acids. A fully biologically active molecule has a molecular mass of 24.6 kDa. The apparent molecular mass of the protein is approximately 29 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
SpeciesHuman
Amino acid sequenceMTQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPYIREFEDPRDAPPPTRAETREERMERKRREKIERRQQEVETELKMWDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRSGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRHAccession #: P08621
Molecular Weight24.6kDa. The apparent molecular mass of the protein is approximately 29kDa with 6XHis tag observed by reducing SDS-PAGE.
Purity> 95% as analyzed by SDS-PAGE. The concentration is 0.27mg/ml.
ApplicationELISA, SDS-PAGE, WB
FormulationPurified protein solution after extensive dialysis against PBS,pH 7.4.
EndotoxinLess than 0.2 EU/μg determined by LAL test.
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
BackgroundComponent of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA.; Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.; Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.