BackgroundIL1B belongs to the IL-1 family. Interleukin 1 (IL-1) is a family of polypeptide cytokines consisting of two agonists, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2) encoded by two distinct genes and perform identical biological functions. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response. It is identified as endogenous pyrogens, and is reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Product Information
Expression systemIL-1b, produced in E.coli is a single non-glycosylated polypeptide chain containing 153 amino acids. A fully biologically active molecule has a molecular mass of 17.3 kDa. The apparent molecular mass of the protein is approximately 21 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
SpeciesHuman
Amino acid sequenceAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
Accession #:
P01584
Molecular Weight17.3 kDa. The apparent molecular mass of the protein is approximately 21 kDa with 6XHis tag observed by reducing SDS-PAGE.
Purity> 95% as analyzed by SDS-PAGE. The concentration is 1mg/ml.
FormulationPurified protein solution after extensive dialysis against PBS, pH 7.4.
EndotoxinLess than 0.2 EU/μg determined by LAL test.
Samples are stable for twelve months from date of receipt storing at -20℃. Please avoid repeated freeze-thaw cycles.