YWHAB Polyclonal Antibody
Datasheet
Catalog Number:
E91023Amount:
100ulBackground:
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, has been observed (4). 14-3-3 binding masks specific sequences of the target protein and therefore modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).Species:
RabbitIsotype:
IgGStorage/Stability:
Store at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Altername:
YWHAB;GW128;HS1;KCIP-1 ;14-3-3,Immunogen:
Recombinant protein of human YWHABPurification:
Affinity purificationReactivity:
H M RApplications:
WB IHCMolecular Weight:
6kDaSwiss-Prot No.:
P31946Gene ID:
7529References:
1. Muslin, A.J. and Xing, H. (2000) Cell Signal 12, 703-9. 2. Mackintosh, C. (2004) Biochem. J. 381, 329-42. 3. Dougherty, M.K. and Morrison, D.K. (2004) J. Cell Sci. 117, 1875-84. 4. Yaffe, M.B. (2002) FEBS Lett. 513, 53-7. 5. Bridges, D. and Moorhead, G.B. (2004) Sci. STKE 2004, re10.
WB 1:500 - 1:2000
IHC 1:50- 1:200
- Western blot analysis of extracts of variouscell lines, using YWHAB antibody.
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