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TLR4 Mouse Monoclonal Antibody
WB
Price on request
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Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Product TypeMouse monoclonal IgG1, primary antibodies
Species reactivityHuman, Mouse, Rat
Molecular WtPredicted band size: 96 kDa
DescriptionToll-like receptor 4 (TLR4), also designated as CD284 (cluster of differentiation 284), is a key activator of the innate immune response and plays a central role in the fight against bacterial infections. TLR4 is a transmembrane protein of approximately 95 kDa that is encoded by the TLR4 gene. TLR4 belongs to the toll-like receptor family which is representative of the pattern recognition receptors (PRR), so named for their ability to recognize evolutionarily conserved components of microorganisms (bacteria, viruses, fungi and parasites) called pathogen-associated molecular patterns (PAMPs). The recognition of a PAMP by a PRR triggers rapid activation of the innate immunity essential to fight infectious diseases.
Positive controlJurkat cell lysate, THP-1 cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate.
Subcellular LocationCell membrane, Early endosome, Cell projection, ruffle.
Database linksSwissProt: O00206 Human | Q9QUK6 Mouse | Q9QX05 Rat
Recommended DilutionsWB 1:1,000
Storage Buffer1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
PurityProtein A affinity purified.
ImmunogenRecombinant protein within mouse TLR4.
Storage/StabilityI nstruction: Store at +4℃
blot analysis of TLR4 on different lysates with Mouse anti-TLR4 antibody at 1/1,000 1: Jurkat cell lysate (30 µg/Lane). Lane 2: THP-1 cell lysate (30 µg/Lane). Lane 3: lysate (30 µg/Lane). Lane 4: Neuro-2a cell lysate (30 µg/Lane). Lane 5: Rat liver tissue lysate Lane 6: Rat spleen tissue lysate (40 µg/Lane). Lane 7: Rat colon tissue lysate Predicted band size: 96 kDa. Observed band size: 96 kDa. Exposure time: 25 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was
Conjugated Antibody
This product is also available conjugated. Format: E8RT1666-Conjugate. Price: $485 / Size: 100μl.
E8RT1666-FITC$485 / 100μlE8RT1666-Biotin$485 / 100μlE8RT1666-HRP$485 / 100μlE8RT1666-AP$485 / 100μlE8RT1666-AF350$485 / 100μlE8RT1666-AF405M$485 / 100μlE8RT1666-AF488$485 / 100μlE8RT1666-AF514$485 / 100μlE8RT1666-AF532$485 / 100μlE8RT1666-AF555$485 / 100μlE8RT1666-AF568$485 / 100μlE8RT1666-AF405S$485 / 100μlE8RT1666-AF405L$485 / 100μlE8RT1666-AF546$485 / 100μlE8RT1666-AF594$485 / 100μlE8RT1666-AF610$485 / 100μlE8RT1666-AF635$485 / 100μlE8RT1666-AF647$485 / 100μlE8RT1666-AF680$485 / 100μlE8RT1666-AF700$485 / 100μlE8RT1666-AF750$485 / 100μlE8RT1666-AF790$485 / 100μlE8RT1666-APC$485 / 100μlE8RT1666-PE$485 / 100μlE8RT1666-Cy3$485 / 100μlE8RT1666-Cy5$485 / 100μlE8RT1666-Cy5.5$485 / 100μlE8RT1666-Cy7$485 / 100μlE8RT1666-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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