ADGRE1 Rabbit pAb | EnoGene Biotech Co. Ltd

ADGRE1 Rabbit pAb
Datasheet

Catalog Number:
E47R7058
Amount:
100ul
Cite ID#:
EN5553931
Price:
295$
Reactivity:
Human,Mouse
Predicted Reactivity:
Rat,Pig,GuineaPig
Swiss-Prot No.:
Q14246
Form:
Liquid
Altername:
F4/80; Adhesion G protein-coupled receptor E1; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1; EGF like module receptor 1; EGF TM7; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGFTM7; EMR 1; EMR1; EMR-1; EMR1 hormone receptor; EMR1_HUMAN; AGRE1_HUMAN; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3.
Storage/Stability:
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Immunogen:
KLH conjugated synthetic peptide derived from human ADGRE1
Purification:
Affinity purified by Protein A
Applications:
WB:1:500-2000,Flow-Cyt:2μg/Test,ELISA:1:5000-10000
Gene ID:
2015
Subcellular Location:
Cell membrane.
Antibody type:
Polyclonal
Isotype:
IgG
Immunogen range:
701-800/886 aa
Molecular Weight:
95 kDa
Sample:
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- EMR1 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 95 kD
Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,) at 37℃ for 20 min;
Incubation: Anti-EMR1/F4/80 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB() staining
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-ADGRE1 antibody
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.