Antibody products, Antibody development, Antibody Contract Outsourcing,Antibody Services-EnoGene Biotech Co. Ltd

Catalog Number:
E47R1405
Amount:
100ul
Cite ID#:
EN5551480
Price:
295$
Reactivity:
Human,Mouse,Rat
Predicted Reactivity:
Chicken,Dog,Pig,Cow,Zebrafish,Sheep
Swiss-Prot No.:
P15257
Form:
Liquid
Altername:
HNF1A; TCF1; HNF1 alpha; HNF-1 Alpha; Hepatocyte nuclear factor 1 Alpha; Albumin proximal factor; Hepatic nuclear factor 1 alpha; Hepatic nuclear factor 1; Hepatic transcription factor 1 alpha; Hepatic transcription factor 1; HNF 1; HNF 1A; HNF1A; Interferon production regulator factor; LF B1; LF B1 hepatic nuclear factor; LFB 1; LFB1; LFB1 hepatic nuclear factor; Liver specific transcription factor LF B1; Liver specific transcription factor LFB1; Maturity onset diabetes of the young 3; MODY 3; MODY3; TCF 1; TCF1; TCF-1; Transcription factor 1; Transcription factor 1 hepatic; HNF1A_HUMAN.
Storage/Stability:
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Immunogen:
KLH conjugated synthetic peptide derived from human HNF1
Purification:
Affinity purified by Protein A
Applications:
WB:1:500-2000,IHC-P:1:100-500,ICC:1:100,Flow-Cyt:3ug/test,ELISA:1:5000-10000
Gene ID:
6927
Subcellular Location:
Nucleus. [ALTERNATIVE PRODUCTS] Event=Alternative splicing; Named isoforms=3; Name=A; IsoId=P20823-1; Sequence=Displayed; Name=B; IsoId=P20823-2; Sequence=VSP_002250, VSP_002251; Name=C; IsoId=P20823-3; Sequence=VSP_002252, VSP_002253.
Antibody type:
Polyclonal
Isotype:
IgG
Immunogen range:
201-350/628 aa
Molecular Weight:
67 kDa
Sample:
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-HNF1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 67 kD
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, ) at 37°C for 20 min; Antibody incubation with (HNF1A) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HNF1A) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
Blank control:A549.
Primary Antibody (green line): Rabbit Anti-HNF1A antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.