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CDX2 Recombinant Rabbit mAb
Datasheet

Catalog Number:
E47M52348
Amount:
100ul
Cite ID#:
EN5554700
Price:
495$
Reactivity:
Rat,Human
Swiss-Prot No.:
Q99626
Form:
Liquid
Altername:
cau-dal-related homeobox transcription factor 2; Caudal type homeo box 2; Caudal type homeobox protein 2; caudal type homeobox transcription factor 2; CDX 2; CDX 3; CDX3; Homeobox protein CDX-2; Homeobox protein CDX2; CDX2_HUMAN.
Storage/Stability:
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Immunogen:
KLH conjugated synthetic peptide derived from human CDX2
Purification:
Affinity purified by Protein A
Applications:
WB:1:500-2000,IHC-P:1:100-500,ICC:1:50-200,IF:1:50-200
Gene ID:
1045
Subcellular Location:
Nucleus.
Antibody type:
Recombinant
Isotype:
IgG
Molecular Weight:
34 kDa
Western blot analysis of CDX2 on AGS cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Predicted band size: 34 kDa
Observed band size: 40 kDa
ICC staining of CDX2 in LOVO cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CDX2 antibody at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.