ALKBH1 Rabbit Monoclonal Antibody [KD Validated]

Catalog Number:
E46F69441
Amount:
40ul
Cite ID#:
EN5547033
Price:
295$
Gene Name:
ALKBH1
Purification:
The antibody was purified by immunogen affinity chromatography.
Storage Buffer:
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage/Stability:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Swiss-Prot No.:
Q13686
Form:
Liquid
Altername:
ALKBH1; AlkB Homolog 1, Histone H2A Dioxygenase; ABH; ALKBH; HABH; Alpha-Ketoglutarate-Dependent Dioxygenase ABH1; Alkylated DNA Repair Protein AlkB Homolog 1; DNA N6-Methyl Adenine Demethylase ALKBH1; MRNA N(3)-Methylcytidine Demethylase; DNA Oxidative Demethylase ALKBH1; Nucleic Acid Dioxygenase ALKBH1; DNA 6mA Demethylase; DNA Lyase ABH1; AlkB; ABH1; AlkB, Alkylation Repair Homolog 1 (E. Coli); AlkB, Alkylation Repair Homolog (E. Coli); TRNA N1-Methyl Adenine Demethylase; AlkB, Alkylation Repair Homolog 1; Alkylation Repair, AlkB Homolog; DNA Demethylase ALKBH1; EC 1.14.11.51; EC 1.14.11.33; EC 1.14.11.-; EC 4.2.99.18; ALKB
Immunogen:
A synthesized peptide derived from human ALKBH1
Reactivity:
Human, Mouse, Rat
Applications:
Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Background:
Gene Name: ALKBH1, UniProt Entry: Q13686, NCBI Gene Entry: 8846
Source:
Rabbit
Antibody type:
Rabbit monoclonal antibody
Isotype:
Rabbit IgG
Molecular Weight:
Predicted, 44 kDa, observed, 43 kDa
Recommended Dilutions:
Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:2,000, Immunocytochemistry (IC): 1:1,000
Human Gene Id:
8846
Western blotting analysis using anti-ALKBH1 antibody (E46F69441). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALKBH1 antibody (E46F69441, 1:5000) and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using ECL Substrate Kit .
Western blotting analysis using anti-ALKBH1 antibody (E46F69441). ALKBH1 expression in wild type (WT) and ALKBH1 shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ALKBH1 antibody (E46F69441, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using ECL Substrate Kit .
Flow cytometric analysis of ALKBH1 expression in HepG2 cells using ALKBH1 antibody (E46F69441, 1:2,000). Green, isotype control; red, ALKBH1.
Immunocytochemical staining of HepG2 cells with ALKBH1 antibody (69441, 1:1,000) . Nuclei were stained blue with DAPI; ALKBH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: medium. Scale bar: 20 μm.