Aquaporin 1 Rabbit Monoclonal Antibody [KD Validated]

Aquaporin 1 Rabbit Monoclonal Antibody [KD Validated]
Datasheet

Catalog Number:
E46F68661
Amount:
40ul
Cite ID#:
EN5547050
Price:
295$
Gene Name:
AQP1
Purification:
The antibody was purified by immunogen affinity chromatography.
Storage Buffer:
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage/Stability:
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Swiss-Prot No.:
P29972
Form:
Liquid
Altername:
AQP1; Aquaporin 1 (Colton Blood Group); CHIP28; Aquaporin 1 (Channel-Forming Integral Protein, 28kDa, CO Blood Group); Water Channel Protein For Red Blood Cells And Kidney Proximal Tubule; Urine Water Channel; Aquaporin-CHIP; Aquaporin-1; CO; Aquaporin 1 (Channel-Forming Integral Protein, 28kDa); Channel-Like Integral Membrane Protein, 28-KDa; Aquaporin 1, Colton Blood Group Antigen; Colton Blood Group Antigen; Bloodgroup CO Protein; Colton Blood Group; Aquaporin 1; AQP-CHIP; AQP-1
Immunogen:
A synthesized peptide derived from human AQP1
Reactivity:
Human, Mouse
Applications:
Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Background:
Gene Name: AQP1, UniProt Entry: P29972, NCBI Gene Entry: 358
Source:
Rabbit
Antibody type:
Rabbit monoclonal antibody
Isotype:
Rabbit IgG
Molecular Weight:
Predicted, 29 kDa, observed, 28 kDa
Recommended Dilutions:
Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:2,000, Immunocytochemistry (IC): 1:1,000
Human Gene Id:
358
Western blotting analysis using anti-Aquaporin 1 antibody (E46F68661). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aquaporin 1 antibody (E46F68661, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using ECL Substrate Kit .
Western blotting analysis using anti-Aquaporin 1 antibody (E46F68661). Aquaporin 1 expression in wild type (WT) and aquaporin 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Aquaporin 1 antibody (E46F68661, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using ECL Substrate Kit .
Flow cytometric analysis of Aquaporin 1 expression in C2C12 cells using Aquaporin 1 antibody (E46F68661, 1:2,000). Green, isotype control; red, Aquaporin 1.
Immunocytochemical staining of C2C12 cells with Aquaporin 1 antibody (E46F68661, 1:1,000). Nuclei were stained blue with DAPI; Aquaporin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.