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Phospho-CDK1/2/3(T14) Rabbit Monoclonal Antibody
Datasheet   

  • Catalog Number:

    E46F1641

  • Amount:

    40ul

  • Cite ID#:

    EN5546159

  • Price:

    225$

  • Gene Name:

    CDK1

  • Purification:

    The antibody was purified by immunogen affinity chromatography.

  • Storage Buffer:

    Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

  • Storage/Stability:

    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

  • Swiss-Prot No.:

    P06493

  • Form:

    Liquid

  • Altername:

    CDK1; Cyclin Dependent Kinase 1; CDC28A; CDC2; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; Cyclin-Dependent Kinase 1; P34 Protein Kinase; P34CDC2; Cell Cycle Controller CDC2; EC 2.7.11.22; EC 2.7.11.23; CDKN1

  • Immunogen:

    A synthesized peptide derived from human Phospho-CDK1/2/3 (T14)

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    Western Blotting (WB); Flow Cytometry (FCM); Immunocytochemistry (IC)

  • Background:

    Gene Name: CDK1, UniProt Entry: P06493, NCBI Gene Entry: 983

  • Source:

    Rabbit

  • Antibody type:

    Rabbit monoclonal antibody

  • Isotype:

    Rabbit IgG

  • Molecular Weight:

    Predicted, 34 kDa, observed, 34 kDa

  • Recommended Dilutions:

    Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:2,000, Immunocytochemistry (IC): 1:1,000

  • Human Gene Id:

    983

  • Western blotting analysis using anti-Phospho-CDK1/2/3 (T14) antibody (E46F1641). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-CDK1/2/3 (T14) antibody (E46F1641, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using ECL Substrate Kit .

  • Flow cytometric analysis of Phospho-CDK1/2/3 (T14) expression in HepG2 cells using Phospho-CDK1/2/3 (T14) antibody (E46F1641, 1:2,000). Green, isotype control; red, Phospho-CDK1/2/3 (T14).

  • Immunocytochemical staining of HepG2 cells with Phospho-CDK1/2/3(T14) antibody (E46F1641, 1:1,000). Nuclei were stained blue with DAPI; Phospho-CDK1/2/3(T14) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

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  • Amount:40ul
  • Price:225.00$

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