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ICAM-1 Protein, Rat, Recombinant (His Tag)
Datasheet   

  • Catalog Number:

    E45R53752M2-100

  • Amount:

    100 ug

  • Cite ID#:

    EN5423842

  • Price:

    1223$

  • Type:

    Recombinant Proteins

  • Research Field:

    Neuroinflammation

  • Protein length:

    A DNA sequence encoding the rat ICAM1 (Q00238-1) extracellular domain (Met 1-Thr 493) was expressed, fused with a polyhistidine tag at the C-terminus.

  • Expression Host:

    HEK293 Cells

  • Species:

    Rat

  • Amino acid sequence:

    Met1-Thr493

  • Tag:

    C-His

  • Molecular Weight:

    The recombinant rat ICAM1 comprises 477 amino acids and predicts a molecular mass of 52.7 kDa. The apparent molecular mass of the rat ICAM1 is approximately 70-75 kDa in SDS-PAGE under reducing conditions due to glycosylation.

  • Activity:

    Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When cells are added to rat ICAM1 coated plates (12.5 μg/mL, 100 μL/well), approximately > 30% cells will adhere specifically.

  • Endotoxin:

    < 1.0 EU per μg of the protein as determined by the LAL method.

  • Gene Name:

    intercellular adhesion molecule 1

  • Gene symbol:

    ICAM1

  • Swiss-Prot No.:

    Q00238-1

  • Function:

    Diagnostic proteins, CD Molecule proteins, Biomarker Proteins

  • Summary:

    Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.

  • Form:

    Lyophilized

  • Purity:

    > 97 % as determined by SDS-PAGE.

  • Storage/Stability:

    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
    Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

  • Storage Buffer:

    Lyophilized from sterile PBS, pH 7.4.
    Please contact us for any concerns or special requirements.
    Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
    Please refer to the specific buffer information in the hard copy of CoA.

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  • Amount:100 ug
  • Price:1223$

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ICAM-1 Protein, Rat, Recombinant (His Tag) | EnoGene Biotech Co. Ltd