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Rabbit anti DDDDK-Tag pAb
IP (Oryza sativa, Homo sapiens, Danio rerio)
WB (Mus musculus, Homo sapiens, Sus scrofa)
ChIP (Homo sapiens)
IF (Homo sapiens, Sus scrofa)
Co-IP (Homo sapiens)
WB (Mus musculus, Homo sapiens, Sus scrofa)
ChIP (Homo sapiens)
IF (Homo sapiens, Sus scrofa)
Co-IP (Homo sapiens)
$305/ 100 µl
QuantityTotal: $305
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Cite ID#EN5516899
TypeTag and Loading Control Antibodies
HostRabbit
Positive control293T
IsotypeIgG
SummaryFLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Price305$
ImmunogenA synthetic peptide corresponding to DDDDK tag.
PurificationAffinity purification
Storage/StabilityStore at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
DilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:100
All Names
AlternameDDDDK; DDDDK tag; DDDDK-tag
Conjugated Antibody
This product is also available conjugated. Format: E45R28863N-Conjugate. Price: $485 / Size: 100μl.
E45R28863N-FITC$485 / 100μlE45R28863N-Biotin$485 / 100μlE45R28863N-HRP$485 / 100μlE45R28863N-AP$485 / 100μlE45R28863N-AF350$485 / 100μlE45R28863N-AF405M$485 / 100μlE45R28863N-AF488$485 / 100μlE45R28863N-AF514$485 / 100μlE45R28863N-AF532$485 / 100μlE45R28863N-AF555$485 / 100μlE45R28863N-AF568$485 / 100μlE45R28863N-AF405S$485 / 100μlE45R28863N-AF405L$485 / 100μlE45R28863N-AF546$485 / 100μlE45R28863N-AF594$485 / 100μlE45R28863N-AF610$485 / 100μlE45R28863N-AF635$485 / 100μlE45R28863N-AF647$485 / 100μlE45R28863N-AF680$485 / 100μlE45R28863N-AF700$485 / 100μlE45R28863N-AF750$485 / 100μlE45R28863N-AF790$485 / 100μlE45R28863N-APC$485 / 100μlE45R28863N-PE$485 / 100μlE45R28863N-Cy3$485 / 100μlE45R28863N-Cy5$485 / 100μlE45R28863N-Cy5.5$485 / 100μlE45R28863N-Cy7$485 / 100μlE45R28863N-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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