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DDDDK-Tag Rabbit mAb
WB (Arabidopsis thaliana, Mus musculus, Homo sapiens, Other, Oreochroms mossambcus)
IP (Arabidopsis thaliana, Homo sapiens, Mus musculus, Other)
IHC (Mus musculus)
IF (Mus musculus)
ChIP (Arabidopsis thaliana)
IP (Arabidopsis thaliana, Homo sapiens, Mus musculus, Other)
IHC (Mus musculus)
IF (Mus musculus)
ChIP (Arabidopsis thaliana)
$373/ 100 µl
QuantityTotal: $373
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Product Information
Cite ID#EN5520360
TypeTag and Loading Control Antibodies
HostRabbit
Positive control293T
IsotypeIgG
SummaryFLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
FormLiquid
Storage BufferBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Price373$
ImmunogenA synthetic peptide corresponding to DDDDK tag.
PurificationAffinity purification
Storage/StabilityStore at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:500
IF 1:50 - 1:500
IP 1:50 - 1:500
IHC 1:50 - 1:500
IF 1:50 - 1:500
IP 1:50 - 1:500
All Names
AlternameDDDDK; DDDDK tag; DDDDK-tag
Categories by research area
Categories by product type
- Antibodies
- Primary Antibodies
- Tag antibodies
- Internal control antibodies
- Pathological (Immunohistochemistry, IHC) antibodies
- Cell Signal Transduction Research Antibodies
- Secondary Antibody
- Antibody Sampler Kits
- ELISA Kits
- Recombinant Proteins
- Fluorescence Reagents
- Signal Inhibitors and Activators
- Transfection Reagent
- Cell biology kits
- Overexpression Cell Lines
- Cell Lysates
- Purification Resins
- 2019-nCoV & Monkeypox