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Phospho-ACK1 (Y518) Antibody
Datasheet   

  • Catalog Number:

    E45R05212G-4

  • Amount:

    50 µl

  • Cite ID#:

    EN5472929

  • Price:

    295$

  • Type:

    Rabbit Polyclonal Antibody

  • Host:

    Rabbit

  • Reactivity:

    H

  • Immunogen:

    This ACK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y518 of human ACK1.

  • Purification:

    Affinity chromatography purified.

  • Gene Name:

    TNK2

  • Gene ID:

    10188

  • Swiss-Prot No.:

    Q07912

  • Function:

    Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP (PubMed:20110370).

  • Summary:

    Tissue Location:
    The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.

  • Form:

    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

  • Storage/Stability:

    For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

  • Applications:

    DB

  • Dilution:

    DB--1:500

  • Altername:

    Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2, TNK2, ACK1

  • Synonyms:

    Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2, TNK2, ACK1

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  • Amount:50 µl
  • Price:295$

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Phospho-ACK1 (Y518) Antibody | EnoGene Biotech Co. Ltd